Fig. 1 | Nature Communications

Fig. 1

From: Synthetic ligands for PreQ1 riboswitches provide structural and mechanistic insights into targeting RNA tertiary structure

Fig. 1

Small molecule microarray screening. a Sequence of the Cy5-labeled aptamer domain of the BsPreQ1 riboswitch (5′-Cy5-BsPreQ1-RS) used for SMM screening. The conformational change induced by PreQ1 binding is also shown. b SMM images for the hit compound 1 (compound is printed in duplicate). Spot diameter (white circle) is 150 µm. c Selectivity of the hit compound across various oligonucleotides screened using the SMM platform (Supplementary Table 2) as measured by composite Z-score. Horizontal line corresponds to a “hit” cutoff of 3. d Sequence alignment of Bs, Ss, and TtPreQ1 riboswitch aptamers. Conserved nucleotides are marked by asterisks. Secondary structure of the class I PreQ1 riboswitch aptamers is below the alignment and base-pairing interactions are indicated by colored bars

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