Fig. 1

Hepatocyte Gps2 depletion in mice improves MCD-induced fibrosis. a Flowchart representing the Gps2 floxed mice generation and LKO strategy. b Fed and fasted ketone body analysis, fed n = 5, fasted n = 11, one-way ANOVA followed by Tukey’s test. c Mitochondrial respiration reflected by OCR levels in BSA or BSA-palmitate treated control (shLuc) and GPS2 knockdown (shGPS2) AML12 cell lines (left panel) and area under the curve calculations (right panel), n = 13, one-way ANOVA followed by Tukey’s test. (d) Serum ALT and (e) AST activity in chow diet (CD) and MCD-treated WT and LKO mice, n = 4 in CD and n = 8 in MCD, one-way ANOVA followed by Tukey’s test. (f) H&E staining; (g) liver triglyceride, n = 6 in WT CD, n = 7 in LKO CD, n = 7 in WT MCD and n = 5 in LKO MCD; (h) surius red staining; (i) liver sirius red staining quantification, n = 8 in WT CD, n = 6 in LKO CD, n = 8 in WT MCD, n = 13 in LKO MCD; (j) liver hydroxyproline test, n = 4 in CD groups, n = 6 in WT MCD, n = 7 in LKO MCD; (k) F4/80 staining; (l) F4/80 staining quantification, n = 8 in WT CD, n = 6 in LKO CD, n = 5 in WT MCD, n = 6 in LKO MCD; and (m) qPCR analysis of gene expression, n = 5–7 in each groups, one-way ANOVA followed by Tukey’s test. All data are represented as mean ± s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001