Fig. 5
GPS2 cooperates with NCOR to repress target gene expression. a, b ChIP-seq tracks representing H3K27ac at (a) Pdk4 and (b) Cyp4a14 loci in Gps2, Ncor and Smrt LKO and respective WT mouse livers. Percentage of increase represents logFC of each peak and is calculated from biological duplicates (for Gps2 LKO versus WT) or triplicates (for Ncor or Smrt LKO versus WT). c, d ChIP-seq tracks representing NCOR, GPS2, PPARα, and Pol II recruitment in Pparα KO, Ncor LKO, Gps2 LKO and respective WT livers at (c) Pdk4 and (d) Cyp4a14 loci. Percentage of increase represents logFC of each peak and is calculated from biological triplicates. e Boxplot representing average logFC of co-localized GPS2 peaks in Ncor LKO and Pparα KO versus WT livers (n = 3 in each group). f, g MA plots showing the average logFC of GPS2 peaks between (f) Ncor LKO versus WT; and (g) Pparα KO versus WT livers, against average logCPM. UP, UN, and DN represents upregulated, unchanged, and downregulated peaks, respectively. All data are represented as mean ± s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001
