Fig. 4
From: Miniaturised interaction proteomics on a microfluidic platform with ultra-low input requirements
On-chip AP-MS using antibodies. a, b Volcano plot of SMC1A-GFP enriched using a GFP antibody (a) and an antibody against native SMC3 protein (b). ID refers to the total number of proteins reported and FE stands for fold enrichment. Dotted gray lines represent statistical cut-offs. Black dots identify significant proteins; red dots are used for known interactors of the bait. EMG1 is a known contaminant33. The volcano plot in panel a presents n = 1 biologically independent experiment, each comprised of n = 3 technical replicates for PI-GFP and control extract. The volcano plot in panel (b) presents n = 2 biological replicates, each comprised of n = 3 technical replicates for PI and control extract
