Fig. 7
Wnt5a is a spatial cue and Yap/Taz and Piezo1 act downstream of Wnt5a. a Sox:Cre;Z/Wnt5a embryos expressed Wnt5a beyond the normal expression domains by in situ hybridisation. b OPT showing a short and wide mandibular arch in a 21 somite Sox:Cre;Z/Wnt5a embryo. c F-actin and pMLC biases were diminished in 20–21 somite Sox:Cre;Z/Wnt5a embryos. d Proportion of cells exhibiting nuclear YAP in the Wnt5a−/− mandibular arch. Scale bars: 20 μm. e OPT showing phenotype of T:Cre;Yapf/+;Tazf/f mandibular arch. f Orientation of mesenchymal cell intercalations in the sagittal plane of mandibular arch mid-regions of WT and T:Cre;Yapf/+;Tazf/f embryos. Intercalation angles were binned into one of 4 arcs of 90o each: anterior, posterior, proximal and distal (n for WT = 48, n for mutants = 42, where n = No. of groups of 5–7 cells). g Multiple vinculin force curves showed no difference in the variance (amplitude) of fluorescence lifetime values of Yapf/+;Tazf/f and Tcre;Yapf/+;Tazf/ middle mesenchymal cells (p = 0.185, ANOVA, n = 16 cells in each of two 20–21 somite embryos per condition). h PIEZO1 immunostain intensity was diminished in the Wnt5a−/− mandibular arch. Scale bars: 20 μm. i The 21 somite Piezo1−/− mutant mandibular arch partially phenocopied that of Wnt5a−/− with a short and broad mid-portion. j YAP/TAZ and PIEZO1 partially mediate actomyosin bias and cortical oscillation amplitude, respectively, downstream of WNT5A to orient and promote mesenchymal cell intercalations. Source data are provided as a Source Data file
