Fig. 2
From: Single-molecule characterization of extrinsic transcription termination by Sen1 helicase
ATP-dependent removal of stalled Pol II by Sen1. a Time-trace showing successive extension increases as Pol II molecules accumulate on the DNA without terminating. b Time-trace showing a single Pol II can be removed by full-length Sen1 in the presence of ATP. Black arrows indicate moment of addition of indicated component. For the sake of clarity, DNA extension during additions is not shown. c Time-trace showing Pol II removal by Sen1 HD does not take place in the absence of ATP, but takes place in the presence of ATP. d Time-trace showing Sen1 HD does not remove stalled Pol II if provided with ATP-γ-S, a slowly hydrolyzable ATP analog
