Fig. 3 | Nature Communications

Fig. 3

From: Mitochondria-specific drug release and reactive oxygen species burst induced by polyprodrug nanoreactors can enhance chemotherapy

Fig. 3

Cancer cell targeting property of DT-PNs. a CLSM images of 4T1 cells (integrin positive) and MCF-7 cells (integrin negative) after incubating with RhB-labelled DT-PNs under different treatments for 2 h at 37 °C. For the pre-blocking experiment, cells were pretreated with 2 μM free cRGD for 30 min before incubation with DT-PNs (scale bar, 50 μm). b, c Flow cytometry analysis and the statistical analysis in a. The mean value was calculated by the t test (mean ± s.e.m. n = 3). ***p < 0.001, compared with the indicated group. d Flow cytometry analysis of 4T1 cells upon 6 h treatment with RhB-labelled polyprodrug nanoparticles with diverse targeting moieties, including non-targeting nanoparticles (NT-PNs) from the assembly of PDMA-b-PCPTSM and PDMA-b-P(CPTSM-co-RhB); TPP-PNs from the assembly of TPP-PDMA-b-PCPTSM and PDMA-b-P(CPTSM-co-RhB), cRGD-PNs from the assembly of cRGD-PDMA-b-P(CPTSM-co-RhB) and PDMA-b-PCPTSM, and the final resultant DT-PNs. e Statistical analysis of the mean fluorescence intensity in b. The mean value was calculated by the t test (mean ± s.e.m. n = 3). **p < 0.01, ***p < 0.001, compared with the indicated group

Back to article page