Fig. 2

C. elegans reversal behavior is controlled by CEPsh glia-expressed glt-1. a Reversal rates in wild type (WT, black circles; n = 9 movies), CEPsh-glia-ablated (black boxes; n = 9 movies), glt-1(ok206) (black triangles; n = 9 movies), eat-4(ky5) (open circles; n = 8 movies), eat-4(ky5); CEPsh-glia-ablated (open boxes; n = 8 movies) and eat-4(ky5); glt-1(ok206) (open triangles; n = 7 movies) strains. b A deconvolved optical section. GLT-1 fused to GFP expressed in CEPsh glia using hlh-17 promoter. Synapses are marked by glr-1pro::NLG-1::mCherry. Scale bar, 4 μm. c Cell-specific glt-1(ok206) rescue studies. Reversal rates normalized relative to minimum value of WT controls and maximum value of glt-1 mutants. Wild type (WT, open circles; n = 23 movies), glt-1(ok206) (black boxes; n = 25 movies), GLT-1 transgene expressed in neurons (magenta triangles; eat-4; n = 11), CEPsh glia (hlh-17, green triangles; n = 14), muscles (myo-3, blue diamonds; n = 18). a, c Bars, mean ± SEM, ANOVA Tukey’s HSD post hoc test, **p < 0.005, ***p < 0.0005. Source data are provided as a Source Data file