Fig. 4
GLT-1 inhibits MGL-2-dependent oscillations in glutamate secretion and AVA activation. a Representative traces of spontaneous glutamate (light blue) and calcium (dark blue) dynamics near and in a wild-type AVA neuron, respectively. b As in (a), glt-1(ok206) mutant (glutamate, light red; calcium, dark red). c Time-dependent frequency spectrum of calcium traces of the wild-type animal in (a). d Time-averaged frequency amplitudes from the wavelet spectrum in (c). e, f As in (c, d), glt-1(ok206) mutant. g Mean time-averaged frequency amplitudes for WT (blue line; 20 traces), glt-1(ok206) (magenta line; 22 traces), mgl-2(tm355) (green line; 15 traces) and glt-1(ok206); mgl-2(tm355) (orange line; 17 traces). h Frequency amplitude differences between indicated strain pairs. Shading, areas of significant difference p < 0.05, permutation test from bootstrapped ensembles, n = 104 bootstrap samples. i Representative trace of head motion in nonparalyzed glt-1 mutant animal between forward (positive) and backward (negative) positions. Traces are decorated in green, magenta for increased, decreased calcium signals, respectively. Circles, transition points. Source data are provided as a Source Data file
