Fig. 6
Glutamate spillover induces repetitive reversals via the egl-30/Gαq signaling pathway. a egl-30/goa-1 mutations ameliorate/exacerbate repetitive reversals of glt-1 mutants (WT, open boxes, n = 17, glt-1(ok206), black boxes, n = 12, egl-30(ad806), open circles, n = 7, glt-1(ok206); egl-30(ad806), black circles, n = 10, goa-1(n1134), open triangles, n = 7, glt-1(ok206); goa-1(n1134), black triangles, n = 8 movies). b EGL-30 expression in RIM neurons rescues repetitive reversals (WT, black circles, n = 14, egl-30(ad806), red circles, n = 15, egl-30(ad806); tdc-1pro::EGL-30, blue circles, n = 8, glt-1(ok206), black boxes, n = 12, glt-1(ok206); egl-30(ad806), red boxes, n = 16, glt-1(ok206); egl-30(ad806); tdc-1pro::EGL-30, blue boxes, n = 22 movies). a, b Bars, mean ± SEM, ANOVA Tukey’s HSD post hoc test, n.s., nonsignificant, ***p < 0.0005, **p < 0.005, *p < 0.05. c Mean time-averaged frequency amplitudes for WT (blue line, 20 traces), glt-1(ok206) (magenta line, 22 traces), goa-1(n1134) (light green, 16 traces) and glt-1(ok206); goa-1(n1134) (dark green, 10 traces). d Mean time-averaged frequency amplitude differences between indicated strain pairs. Shading, areas of significant differences p < 0.05, permutation test from bootstrapped ensembles, n = 104 bootstrap samples. Source data are provided as a Source Data file
