Fig. 3 | Nature Communications

Fig. 3

From: Mechanism-based tuning of insect 3,4-dihydroxyphenylacetaldehyde synthase for synthetic bioproduction of benzylisoquinoline alkaloids

Fig. 3

Structural analysis of AAAD and DHPAAS. Comparison of Drosophila melanogaster DDC in complex with PLP (cyan, a and d, PDB ID: 3K40)25, P. somniferum TyDC1 in complex with PLP-tyrosine (green, b and e), and wild-type B. mori DHPAAS in complex with PLP-DOPA (magenta, c and f). Phe79 and Tyr80 are conserved in DHPAAS sequences, whereas this motif is reversed to Tyr79 and Phe80 in typical AAAD, which includes DDC. In the D. melanogaster DDC-PLP complex with no substrate, Phe103 extends inwards towards the PLP cofactor. The bottom three panels show engineered active sites of Phe79Tyr-Tyr80Phe-Asn192His (g, blue), Asn192His (h, dark green) and Phe79Tyr-Tyr80Phe (i, orange) variants of B. mori DHPAAS, all in complex with PLP-DOPA

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