Fig. 3 | Nature Communications

Fig. 3

From: Canonical PRC1 controls sequence-independent propagation of Polycomb-mediated gene silencing

Fig. 3

cPRC1 and H3K27me3 are required for maintenance of reporter gene silencing. a Flow cytometry histograms before and after 6 days of Dox treatment of CRISPR mutant clones isolated from sgRNA-treated cPRC1-mESCs. Percentage indicates fraction of GFP-negative reporter cells. b Percentage of GFP- and BFP-negative cells before and after 6 days of Dox treatment in response to increasing concentrations of Ezh2 inhibition by GSK126. Data are mean ± SD (error bars) of two independent experiments

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