Fig. 2
From: TORC1 modulation in adipose tissue is required for organismal adaptation to hypoxia in Drosophila
Hypoxia suppresses TORC1 signalling via TSC1/2. a Early third instar larvae were transferred from normoxia to hypoxia (5% oxygen). At the indicated times, larvae were then collected, lysed and processed for SDS-PAGE and western blotting using antibodies to phospho-S6K (pS6K) or total eIF2alpha (eIF2α). b Early third instar larvae were transferred from normoxia to different levels of hypoxia (20–1% oxygen) for 1 h. Larvae were then collected, lysed and processed for SDS-PAGE and western blotting using antibodies to phospho-S6K (pS6K) or total eIF2alpha (eIF2α). c Control (da>+) or Rheb overexpressing (da > Rheb) early third instar larvae were either maintained in normoxia (N) or transferred from normoxia to hypoxia (5% oxygen, H) for 1 h. Larvae were then collected, lysed and processed for SDS-PAGE and western blotting using antibodies to phospho-S6K (pS6K) or total eIF2alpha (eIF2α). Quantified band intensities from three independent experiments are shown below the blot. Data represent relative pS6K band intensities corrected for eIF2α (loading control) band intensity. Quantifications were performed using Image J. Data represent mean ± SD. *p < 0.05, Students t-test. d Control (w1118) or tsc1 mutant (tsc1W240X) larvae were either maintained in normoxia (N) or transferred from normoxia to hypoxia (5% oxygen, H) for 1 h. Larvae were then collected, lysed and processed for SDS-PAGE and western blotting using antibodies to phospho-S6K (pS6K) or total eIF2alpha (eIF2α). Quantified band intensities from three independent experiments are shown below the blot. Data represent relative pS6K band intensities corrected for eIF2α (loading control) band intensity. Quantifications were performed using Image J. Data represent mean ± SD. *p < 0.05, Students t-test. e Control (w1118) or sima mutant (sima07607) larvae were either maintained in normoxia (N) or transferred from normoxia to hypoxia (5% oxygen, H) for 1 h. Larvae were then collected, lysed and processed for SDS-PAGE and western blotting using antibodies to phospho-S6K (pS6K) or total S6K. f Control (w1118) or scylla mutant (scylla) larvae were either maintained in normoxia (N) or transferred from normoxia to hypoxia (5% oxygen, H) for 1 h. Larvae were then collected, lysed and processed for SDS-PAGE and western blotting using antibodies to phospho-S6K (pS6K) or total eIF2alpha (eIF2α)
