Fig. 3
From: TORC1 modulation in adipose tissue is required for organismal adaptation to hypoxia in Drosophila
Suppression of TORC1 is required for adaptation to hypoxia. a–d Control (da>+) or Rheb overexpressing (da > Rheb) animals were maintained in either normoxia or hypoxia (5% oxygen) throughout the larval period. a Survival to the pupal stage was measured by calculating the percentage or larvae that developed to pupae for each experimental condition. b The rate of larval development was measured by calculating the percentage of animals that progressed to the pupal stage over time. N = a minimum of four independent groups of animals (50/group). Maintaining TORC1 signalling in larvae during hypoxia led to a further delay in pupation. c Weights of wandering third instar larvae were measured for each experimental condition. Maintaining TORC1 signalling in hypoxia did not increase larval growth. Data are presented as box plots (25%, median and 75% values) with error bars indicating the min and max values. N = 5 groups of larvae per condition. d, e Control (da>+) or Rheb overexpressing (da > Rheb) animals were maintained in either normoxia or hypoxia (5% oxygen) throughout the larval period, before being returned to normoxia at the pupal stage. The percentage of animals that developed to d pharate adults, or e eclosed adults were then calculated. Maintaining TORC1 signalling in larvae during hypoxia led to a subsequent lethality during pupal development. Data are presented as box plots (25%, median and 75% values) with error bars indicating the min and max values. N = five independent groups of animals (50 animals per group) per experimental condition. *p < 0.05, Students t-test. f Control (da>+) or S6KTE overexpressing (da > S6KTE) animals were maintained in either normoxia or hypoxia (5% oxygen) throughout the larval period, before being returned to normoxia at the pupal stage. The percentage of animals that developed to adults was then calculated. Data are presented as box plots (25%, median and 75% values) with error bars indicating the min and max values. N = a minimum of four independent groups of animals (50 animals per group) per experimental condition. *p < 0.05, Students t-test
