Fig. 1
From: Competitive endogenous RNA is an intrinsic component of EMT regulatory circuits and modulates EMT
FOXP1 is a critical inducer of EMT in A549 cells. a Quantitative reverse transcription polymerase chain reaction (qRT-PCR) results showing the levels of gene expression in A549 cells during TGF-β-induced EMT after silencing FOXP1 expression using specific shRNA. n = 3. b Immunoblotting analysis of the protein abundance of indicated genes in A549 cells undergoing EMT after treatment with an shRNA targeting FOXP1. c A549 cells undergoing TGF-β-induced EMT were treated with an shRNA targeting FOXP1 and subjected to a migration assay (upper panel) and an invasion assay (lower panel). Scale bars: 100 μm. d The migrated or invaded cells were quantified (bar charts). n = 6. e Morphology of A549 cells overexpressing FOXP1 or an empty control vector. f Immunoblotting analysis of the protein abundance of indicated genes in A549 cells overexpressing FOXP1 or an empty control vector. g A549 cells overexpressing FOXP1 or an empty control vector were subjected to a migration assay (upper panel) and an invasion assay (lower panel). Scale bars: 100 μm. h The migrated or invaded cells were quantified (bar charts). n = 6; error bars indicate the means ± s.d. *p < 0.01, determined using the two-tailed Student’s t test. Source data are provided as a Source Data file
