Fig. 4
From: Competitive endogenous RNA is an intrinsic component of EMT regulatory circuits and modulates EMT
FN1 is a functional ceRNA for miR-200c to regulate EMT. a Absolute quantification of the numbers of FN1 mRNA and miR-200c transcripts during TGF-β-induced EMT in MCF10A cells. b Graph showing the dynamics of modeled 8-mer miR-200c binding site occupancy during TGF-β-induced EMT in MCF10A cells using pictar-based predictions. c Graph showing the level of indicated RNAs in MCF10A cells during TGF-β-induced EMT subjected to indicated treatments. d Same as (c) for immunoblotting analysis. e Graph showing the level of indicated RNAs in MCF10A cells during TGF-β-induced EMT subjected to indicated treatments, using cells whose DICER1 has been knocked down. f Same as (e) for immunoblotting analysis. g Graph showing the level of indicated RNAs in MCF10A cells during TGF-β-induced EMT subjected to indicated treatments, using cells whose conserved miR-200c binding site in FN1 3′UTR has been mutated with CRISPR-Cas9. h Same as (g) for immunoblotting analysis. n = 3; error bars indicate the means ± s.d. *p < 0.01, determined using a two-tailed Student’s t test. Source data are provided as a Source Data file
