Fig. 7
From: Staufen2-mediated RNA recognition and localization requires combinatorial action of multiple domains
Model of the molecular recognition of dsRNA by mStau2. a dsRNA recognition by the mStau2 tandem domain dsRBD1–2. dsRBD2 binds dsRNA promiscuously with moderate affinity and slides along the stem. Through this sliding, dsRBD2 positions dsRBD1 close to the dsRNA. When a suitable dsRNA structure is reached, dsRBD1 also binds, thereby strongly increasing the affinity of the tandem domain to dsRNA. b Next to the tandem domain dsRBD 1–2, dsRBD 3–4 acts in a similar way. Here, dsRBD4 does the first promiscuous binding with moderate affinity. When dsRBD2 and dsRBD4 position dsRBD1 and dsRBD3, respectively, close to a suitable dsRNA, the respective domains also bind the dsRNA, thereby increasing affinity. Only when suitable dsRNA binding sites for both tandem domains are in sufficient spatial proximity can all four dsRBDs be bound and form a stable complex with the RNA target
