Fig. 3
From: Retrieving high-resolution information from disordered 2D crystals by single-particle cryo-EM
Conformational continuum of MloK1 from 2D crystals. All the maps were obtained from one 2D crystal dataset using signal subtraction and 3D classification after consensus refinement. The consensus model was flexibly fitted and refined into each 3D class. The CNBD is colored yellow and the TMD is colored blue, while the maps are shown as transparent gray surfaces. The map threshold for each class was calculated such that all iso-surfaces enclose the same volume. The pale blue dashed line indicates the approximate position of the lipid bilayer. Conformational variations among the classes primarily change the height of the CNBDs in relation to the membrane slab and their tilt in relation to the TMD and the VSD. C4 symmetry was applied. Scale bar: 25 Å
