Fig. 2

NALP2, MDA5, and A20 bind the WDD of ATG16L1 in co-precipitation assays. a–c HEK-293T cells were transfected with the indicated constructs (WDD, residues 320–607 of ATG16L1; ΔWDD, 1–299), lysed 36 h after transfection and subjected to GST immunoprecipitation using agarose beads coupled to glutathione (IP: immunoprecipitation; TL: total lysate). Shown are Western-blots against the indicated molecules