Fig. 1 | Nature Communications

Fig. 1

From: Spy&Go purification of SpyTag-proteins using pseudo-SpyCatcher to access an oligomerization toolbox

Fig. 1

Overview of Spy&Go. SpyTag/SpyCatcher interact irreversibly by spontaneous isopeptide bond formation, promoted by E77. SpyTag purification requires the generation of reversible interaction with SpyDock in 3 steps: blocking reaction, enhancing efficiency of SpyTag binding and precise immobilization on resin. Spy&Go then enables purification of SpyTag-fusions, setting the stage for modular oligomerization or multimerization

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