Fig. 4
From: Spy&Go purification of SpyTag-proteins using pseudo-SpyCatcher to access an oligomerization toolbox
Spy&Go from bacterial expression. a SpyTag-MBP was purified from E. coli clarified lysate by Spy&Go. Protein: input SpyTag-MBP protein. T: total pooled elutions. Purity of T was determined by densitometry (right); gray represents background lane intensity (mean ± 1 s.d., n = 3). b Ni-NTA purification of SpyTag-MBP from the same lysate via its His-tag. c Spy&Go purification of scPvuII-SpyTag (SpyTag at an internal loop, shown schematically) from bacterial lysate. d Spy&Go purification of the nanobody αDR5-SpyTag (C-terminal fusion) from bacterial lysate. All fractions were analyzed by SDS-PAGE with Coomassie staining
