Fig. 5 | Nature Communications

Fig. 5

From: Spy&Go purification of SpyTag-proteins using pseudo-SpyCatcher to access an oligomerization toolbox

Fig. 5

Spy&Go from mammalian expression. a HEK293T cells were transfected with the extracellular region of EpCAM fused to SpyTag and a His-tag (EpCAM-SpyTag). EpCAM-SpyTag was purified from the clarified cell supernatant using Spy&Go. Fractions were analyzed by SDS-PAGE with Coomassie staining. T: total pooled elutions. Resin: resin post-elution. b Ni-NTA purification of EpCAM-SpyTag as in a. c Spy&Go purification of CyRPA-SpyTag from Expi293HEK cells as in a

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