Fig. 6
From: Spy&Go purification of SpyTag-proteins using pseudo-SpyCatcher to access an oligomerization toolbox
Construction of oligomeric toolbox. a Spy Oligomerization Toolbox from establishing a panel of coiled coils with valency from 2 to 7 for fusion to SpyCatcher002. The coiled coils are presented with each color representing one chain and the N-terminus colored as a blue ball. The C-terminus of SpyCatcher002, where it will be linked to the coiled coil, is colored as a red ball. b DLS to show assembly of each SpyCatcher002-oligomer, with hydrodynamic radius (Rh) (mean ± 1 s.d., n = 10) for each assembly. c SEC-MALS to show assembly of each SpyCatcher002-oligomer. The peak shows the normalized absorbance units (AU) at 280 nm of the SpyCatcher002-oligomer species from SEC. The horizontal line shows the distribution of molar mass (g/mol) of the species in the peak from MALS. Expected and observed molecular weight (Mr) is shown alongside, with error bars representing the uncertainty in fit to the molar mass curve
