Fig. 7
From: Spy&Go purification of SpyTag-proteins using pseudo-SpyCatcher to access an oligomerization toolbox
Oligomer panel tested for cancer cell killing. a Cartoon depicting depletion of free SpyTag-ligand using Spy&Go resin. b Coupling of SpyTag-fusion to coiled coil series. αDR5-SpyTag was incubated with each SpyCatcher002-coiled coil and analyzed by SDS-PAGE with Coomassie staining. In Recaptured lanes, excess αDR5-SpyTag was removed from the coiled coil conjugate by an additional passage through Spy&Go resin. c Dose-response curve of MDA-MB-231 cell viability when treated with different concentrations of αDR5 conjugated to each SpyCatcher002-oligomer platforms. The line at 50% cell viability shows the cut-off for EC50 calculation. The x-axis is normalized to the concentration of αDR5 monomer. Error bars represent mean ±1 s.d., n = 2. d MDA-MB-231 viability upon incubation as in c with the building blocks of αDR5 alone or coiled coils alone. Error bars represent mean ± 1 s.d., n = 3
