Fig. 1
From: Discovery of processive catalysis by an exo-hydrolase with a pocket-shaped active site
Glc trapped in native HvExoI is bound non-covalently in the 4C1 conformation. a HPLC chromatogram of material extracted from crystals (solid line), mother liquor (dashed) and mother liquor augmented with authentic Glc (dotted). Arrow points to the peak of authentic Glc in augmented mother liquor. Fractions (left right arrow) were pooled for GC/MS analysis. b Total ion chromatograms obtained by GC/MS of HPLC-eluted materials (6–7.3 min) containing alditol acetates. Materials from crystals (top), mother liquor (middle) and mother liquor augmented with Glc (bottom). Myo-inositol (20 ng) served as an internal standard (top and middle panels). Numbers near peaks indicate retention times. Substance eluted in 17.01 min peak corresponds to a contaminating plasticiser. Material extracted from crystals and eluting at 23.26 min (top panel) is identical to that of glucitol hexa-acetate at 23.26 min (bottom panel) on a Prevail Carbohydrate ES column (peaks indicated by asterisks). c Fragmentation mass spectra of glucitol hexa-acetate: top, material extracted from crystals; bottom glucitol hexa-acetate eluting at 23.26 min
