Fig. 7
From: Discovery of processive catalysis by an exo-hydrolase with a pocket-shaped active site
R158A/E161A of HvExoI and with perfused Glc or G6SG-OMe. a Stereo view of a ligand-free form of R158A/E161A. Two glycerol molecules (carbons: green sticks) at 1.0 occupancies are bound in the −1 and +1 subsites. b Stereo view of R158A/E161A with perfused Glc, which failed to bind, instead two glycerol molecules (carbons: green sticks) are bound at 1.0 occupancies in the −1 and +1 subsites. c Stereo view of R158A/E161A with G6SG-OMe (carbons: orange sticks) at 1.0 occupancy, bound across the −1 and +1 subsites. Separations of less than 3.30 Å from electronegative atoms of active site residues (carbons: grey) and water oxygens, are shown as dashed lines. Derived |2mFobs − DFcalc| electron density maps are contoured at 1σ (blue mesh)
