Fig. 5

Silencing of LNK enhances IFN signaling in melanoma cells. a GSEA analysis of significantly enriched gene expression signatures in A375 melanoma cells with silenced LNK using different CRISPR-Cas9 guide RNA. Cells were either cultured with or without IFN gamma (400 U/ml, 24 h); and gene expression was analyzed using RNA sequencing. NES, normalized enrichment score; FDR, false discovery rate. The color scale indicates the positive (red) or negative (blue) correlation. b Silencing of LNK enhanced the IFN-γ induced (400 U/ml, 24 h) gene expression of MHC class I and II. c Silencing of LNK enhanced the IFN response gene expression in A375 cells. d Real time PCR analysis of IFN induced genes expression in A375 cells with either forced expression (LNK OE) or silencing of LNK (sgLNK), either with or without IFN-γ treatment (2000 U/ml, 24 h). *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; ns, not significant; unpaired two tailed t-test. Error bars represent SD