Fig. 5

The lncRNA-p21 disrupts the PRC2 complex and promotes EZH2 to methylate STAT3. a The qPCR analysis of PRC2 target gene expressions in C4-2 cells after overexpression of lncRNA-p21. b EZH2 protein was immunoprecipitated in C4-2 pWPI and pWPI-lncRNA-p21 cells. The co-immunoprecipitated SUZ12 levels were detected by WB. The SYP and H3K27Me3 levels in input samples were also detected by WB. c The WB analysis of STAT3 methylation and phosphorylation with/without (w/o) overexpression of lncRNA-p21 in C4-2 cells. d The qPCR analysis of STAT3 target genes expressions in C4-2 cells w/o overexpression of lncRNA-p21. e The lncRNA-p21 knockdown can reduce Enz-induced STAT3 methylation. C4-2 pLKO and 2 different sh-lncRNA-p21 transfected cells were treated w/o Enz for 4 days. The STAT3 methylation was detected by WB. f Co-IP assay to detect the interaction between EZH2 and STAT3 w/o overexpression of lncRNA-p21 in C4-2 cells. g Confocal microscopic images of co-localization of EZH2 and STAT3 w/o Enz treatment in C4-2 pLKO and sh-lncRNA-p21 cells. The co-localization of EZH2 and STAT3 was analyzed by Pearsons coefficient (Scale bar = 2 μm). Quantitation on the right. For a, d, g, data are presented as mean ± SD, *p < 0.05, **p < 0.005, N.S. not significant by t-test