Fig. 1

CR9501 potently neutralizes RSV and binds near antigenic site V. a Geometric mean neutralization IC₅₀ values for CR9501 (red), palivizumab (gray), and D25 (blue). Error bars denote standard deviation of geometric means (n = 4 biologically independent experiments with nine technical replicates for CR9501 and palivizumab and n = 1 biologically independent experiment with 27–43 technical replicates for D25). b Two protomers of prefusion F are shown as molecular surfaces colored gray and white. The third protomer is shown as ribbons, with the F₁ subunit colored blue and the F₂ subunit colored green. The Cα atoms of residues identified by shotgun mutagenesis are shown as spheres and colored according to Supplementary Table 1. The inset shows a zoomed view of a 45° rotation about the trimeric axis. A linear depiction of F is shown at the top right, with the F₁ and F₂ subunits colored blue and green, respectively. Regions colored white are absent in the soluble protein (SS signal sequence, TM transmembrane, CT cytoplasmic tail). Glycans are shown as branches, disulfide bonds are shown as black lines, and furin cleavage sites are indicated by black arrows