Fig. 2
From: Critical role of bacterial dissemination in an infant rabbit model of bacillary dysentery
Pro-inflammatory immune responses in the colon of infant rabbits infected with Shigella flexneri. a, b Representative images of hematoxylin- and eosin-stained colonic sections from animals inoculated with phosphate-buffered saline (PBS) (a) or S. flexneri (b). Scale bars, 100 μm. Top, ×10 images; bottom, ×40 images of the boxed area in top images. Arrows and arrowheads indicate heterophils. Insets, zoom-in on the heterophil indicated by arrowheads. c Graph showing histopathology score of heterophil infiltration. Statistical analysis, unpaired Student’s t test. Wild-type (WT) vs. mock, ****P < 0.0001. d Chemokine (interleukin-8 (IL-8) and C-X-C motif chemokine 10 (CXCL10)) and cytokine (IL-1β, IL-6, and tumor necrosis factor-α (TNFα)) gene induction (black bars) in the colon 8 h post inoculation (pi) with S. flexneri with respect to mock-treated animals (PBS, white bars). Overlay dot plots represent individual data points. Error bars represent standard deviation of the mean. Source data are provided as a Source Data file
