Fig. 1
From: The glycosylation design space for recombinant lysosomal replacement enzymes produced in CHO cells
Graphic depiction of gene targeting screen performed in CHO cells with general trend effects on N-glycosylation of α-galactosidase A (GLA). clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) knockout (KO) targeted genes are indicated with their predicted functions. a The general trend effects of KO targeting of glycosyltransferase, glycosylhydrolase, and other related genes known to function in N-glycosylation and mannose 6-phosphate (M6P) tagging are indicated for changes in total sialic acid capping (SA), M6P-tagging (M6P), and exposed terminal mannose (Man), with arrows indicating increase/decrease. b Trend effects of KO targeting of genes encoding enzymes functioning in the dolichol-linked precursor oligosaccharide assembly, receptors involved in trafficking of lysosomal enzymes, and other proteins reported to affect stability of enzymes in the Golgi. Glycan symbols according to SNFG format70
