Fig. 3
From: The glycosylation design space for recombinant lysosomal replacement enzymes produced in CHO cells
Site-specific glycan analyses of α-galactosidase A (GLA) glycoforms produced with combinatorial gene engineering of CHO cells. a–l The two most abundant glycan structures at N-glycosites (N108, N161, and N184) of GLA are shown. Each glycan structure was confirmed by targeted tandem mass spectrometry (MS/MS) analysis. The detailed N-glycan analyses of all GLA glycoforms are shown in Supplementary Fig. 2 together with additional variants. Details regarding the stacking ancestry and sequence analysis are shown in Supplementary Table 2 and Supplementary Data 1
