Fig. 3
From: A conserved CCM complex promotes apoptosis non-autonomously by regulating zinc homeostasis
MPK-2/ERK5 and KLF-3 regulate IR-induced apoptosis downstream of KRI-1. a IR-induced germline apoptosis scored after knock-down of the ERK-5/MAPK pathway genes, mekk-3, mek-5, and mpk-2 in wild type, kri-1(ok1251) and daf-2(e1370) animals (n ≥ 60). b IR-induced germline apoptosis scored in wild type, kri-1(ok1251), and kri-1(ok1251); klf-3(on34) animals (n ≥ 45). c Expression of Pmpk-2::gfp. Images are representative of three independent lines (×100 magnification). Scale bar is 50 μm. d IR-induced germline apoptosis scored in wild type, Pmpk-2::mpk-2 “+array”, and Pmpk-2::mpk-2 siblings that have lost the transgene “−array” (n ≥ 30). e IR-induced germline apoptosis scored in wild type, Pelt-2::mpk-2 “+array”, and Pelt-2::mpk-2 siblings that have lost the transgene “−array” (n ≥ 30). a–e Red line is mean ± standard deviation. *P < 0.05, two-sided, unpaired t-test
