Fig. 1
CSC-associated heterogeneity in GBM. a Inter-patient heterogeneity at the gene expression level for a panel of CSC-associated markers. See also Supplementary Fig. 1. b Pearson analysis did not reveal any significant correlation. c Flow cytometric analysis of tumor cells in GBM PDOXs. Percentage of positive cells (black gate) is indicated vs. negative control and vs. high expressing cells (red gate). See Supplementary Fig. 2 for gating strategy and Supplementary Fig. 3 for more examples. d Marker expression profiles in the genetically heterogeneous PDOX T16 (all tumor (black), pseudodiploid (blue), and aneuploid (red) clones). Separately implanted pseudodiploid (blue) and aneuploid clones (red) adapted the CSC-associated profiles in the xenograft (right). e Multicolor phenotyping. Representative ImageStream data shown for NCH644. Right panel: color code for 16 subpopulations applied in all figures. f Distribution of subpopulations in seven patient-derived GBM cultures (mean %, error bars ommitted for visualization purpose). g Distribution of CSC-associated markers and Ki67 proliferating cells in 3D spheres (scale bar = 100 µm)
