Fig. 5
Transcriptome analysis of ILC2 plasticity. a Flow cytometry of intracellular IL-5, IL-13, IL-17A, and IFN-γ production after restimulation with PMA/ionomycin of blood ILC2s exposed to IL-2; IL-2, IL-1β, and IL-12; IL-2, IL-33, and TSLP; IL-2, IL-1β, IL-23, and TGF-β for 5-7 days. b Quantification of the concentration of IL-5, IL-13, IL-17A, and IFN-γ produced by ILC2s cultured as in a (n = 4–10). Each symbol represents an individual donor and the horizontal lines represent the mean. c Quantification of RORγt-expressing, GATA-3-expressing, and T-bet-expressing blood ILC2s upon exposure to IL-2, IL-1β, and IL-12; IL-2, IL-33, and TSLP; IL-2, IL-1β, IL-23, and TGF-β for 5-7 days by intracellular staining. Each symbol represents an individual donor and the horizontal lines represent the mean (n = 6). d Venn diagram showing the overlap of the number of genes that are significantly up-regulated (red), down-regulated (blue), or contra-regulated (yellow). (red circle) freshly isolated ILC2s versus ILC2s cultured with IL-2, IL-33, and TSLP. (blue circle) freshly isolated ILC2s versus ILC2s cultured with IL-2, IL-1β, IL-23, and TGF-β. (green circle) ILC2s cultured with IL-2, IL-33, and TSLP versus ILC2s cultured with IL-2, IL-1β, IL-23, and TGF-β. e Summary of unbiased clustering. Clusters of the number of genes with significant different expression between blood ILC2s stimulated as in d. f Vulcano plot comparing ILC2s cultured with IL-2, IL-33, and TSLP versus ILC2s cultured with IL-2, IL-1β, IL-23, and TGF-β. The 25 most significantly up-regulated or down-regulated genes are labeled. Data are presented as individual values with mean of two independent experiments (b). *p < 0.05, **p < 0.01, ***p < 0.001 as determined by one way ANOVA. Source data
