Fig. 6
ILC2 stimulations result in unique expression patterns. a Heatmap of genes related to ILC2 signature. b Heatmap of genes related to ILC-related transcription factors. c Heatmap of genes related to ILC-related cytokines. d Heatmap of genes encoding significantly differentially expressed surface markers d. Both z-scores (row-normalized) and absolute expression values are shown. e Heatmap of genes significantly different expressed in ILC2s upon stimulation with IL-2, IL-1β, IL-23, and TGF-β as compared with ILC2s cultured with IL-2, IL-33, and TSLP that are related to the Th17 KEGG pathway. f Quantification of expression of CCR4, CCR6, CCR5 and CXCR6 on freshly isolated blood ILC2s, and upon culture for 5 days with IL-2, IL-33 and TSLP or IL-2, IL-1β, IL-23, and TGF-β (n = 5-8). g Representative flow cytometric analysis of CCR4, CCR6, CCR5, and CXCR6 upon culture as in f. h Representative flow cytometric analysis of KLRG1 expression upon culture as in f. Data are presented as individual values with mean of two to four independent experiments (f). *p < 0.05, **p < 0.01 as determined by one way ANOVA. Source data
