Fig. 7
Transdifferentiated ILC2s enhance neutrophil recruitment. a Quantification of ractions of eosinophils and neutrophils in NP tissues of subjects with (n = 5) or without (n = 6) CF as determined by flow cytometry. b Correlation of ILC2 and NKp44-ILC3 frequencies to eosinophil/neutrophil ratio in CRSwNP (n = 13). c, d Representative flow cytometry analysis (c) and quantification (d) of intracellular expression of IL-17A and TNF-α in blood ILC2s exposed to IL-2, IL-33, and TSLP or IL-2, IL-1β, IL-23, and TGF-β for 5-7 days after PMA/ionomycin (n = 9–10). e Quantification of IL-8 production by NCI-H292 epithelial cells after 48 hr exposure to conditioned medium of ILC2s that were stimulated with IL-2, IL-33, and TSLP; or IL-2, IL-1β, IL-23, and TGF-β for 5–7 days (n = 3–4). f Quantification of IL-8, IL-6, and GM-CSF production by NCI-H292 epithelial cells in the presence or absence of IL-17-blocking antibody upon culture as described in e (n = 3). Data are presented as individual values with mean of two to four independent experiments (d–f). *p < 0.05, **p < 0.01, ***p < 0.001 as determined by one way ANOVA or Student’s t test
