Fig. 2
From: The extracellular gate shapes the energy profile of an ABC exporter
The sybody traps TM287/288 in its OF state. a Sybody Sb_TM#35 is shown as cartoon in gray with the CDR1, 2 and 3 highlighted in yellow, orange and red, respectively. Four aromatic residues (Y33, W52, Y59, and W113) that wedge between TMs 1 and 2 of TM287 (teal) and TMs 5′ and 6′ of TM288 (magenta) are highlighted as sticks. b Inhibition of TM287/288’s ATP hydrolysis by Sb_TM#35, Nb_TM#1 and Nb_TM#2. A non-randomized sybody served as control. The data were fitted with a hyperbolic decay function to determine IC50 values, as well as residual activities. The error bars are standard deviations of technical triplicates. c, d DEER analyses of spin-label pairs introduced to probe the extracellular and intracellular TMDs and the NBDs (c), as well as sybody binding to the transporter (d). DEER traces were recorded in the presence of ATP-EDTA with or without unlabeled Sb_TM#35 (c) or in the presence of ATP-EDTA and spin-labeled Sb_TM#35 (d). The graphs show experimental distance distributions, and vertical dotted lines shown in c highlight changes in the mean distances
