Fig. 3
Mitochondrial autoantigen-based pMHCII-NPs blunt PBC in NOD.c3c4 mice. a Changes in serum TBA and ALT levels [n = 15, four experiments; n = 13, three experiments; and n = 8, two experiments (left to right)]. b Representative liver micrographs (×4 and ×10) of liver sections from Cys-NP-treated vs. PDC166–181/IAg7-NP-treated mice (top) and average scores (bottom) [n = 5, two experiments; n = 20, five experiments; n = 17, four experiments; and n = 6, two experiments (left to right)]. Scale bars: 100 μm. c and d Representative common bile duct (CBD) images (c, top), average CBD scores/diameters (c, bottom), representative livers (d, top) and average liver scores/weight (d, bottom) [n = 5, two experiments; n = 30, seven experiments; n = 19, four experiments; and n = 8, two experiments (left to right)]. e Representative images of treated NOD.c3c4 mice. f Anti-PDC-E2 positive antibody activity levels (PAA, see “Methods” for details) and ANA titers (top), and representative images of Hep2 cells stained with diluted (1:160) sera (bottom) (n = 16, five experiments; n = 13, three experiments; n = 8, two experiments, respectively –left–; n = 7, 8 and 5, 1–3 experiments, respectively –right–). Scale bars: 100 μm. g Percentages of tetramer+ cells in 38–44 week-old mice treated from 24 weeks (n = 6 and 7, respectively, two experiments). h and i Macroscopic (h) and microscopic scores (i) for the mice in g [n = 31 (h) or 25 (i); n = 19 (h) or 7 (i); and n = 8 (h and i) (left to right)]. Data correspond to mean ± SEM. P values were calculated via Mann–Whitney U
