Fig. 5
Liver-specific regulatory network formation. a Cytokine profile of LPS-challenged CD11b+ cells from PCLNs and MLNs. b Cytokine profile of Kupffer cells (only statistically significant analytes shown in a and b) (n = 3/group). c Absolute numbers of B-cells (top) and tetramer+ T-cells (bottom) in PCLNs, ILNs, and liver. Data for B-cells correspond to n = 15 and 19 (PCLN), n = 11 and 11 (ILN), and n = 12 and 16, respectively (liver), from 3–5 experiments/organ. Data for tetramer+ cells correspond to n = 14 and 19 (PCLN), n = 11 and 11 (ILN), and n = 15 and 16, respectively (liver), from 3–5 experiments/organ. d Correlation between numbers of B-cells and tetramer+ cells in the PCLNs, liver, and ILNs of PDC166–181/IAg7-NP-treated mice (n = 19, 16 and 11; 4, 5 and 3 experiments, respectively). e IL-10 secretion by LPS-challenged B-cells from PCLNs, MLNs, and liver (n = 4 and 3 mice/treatment type, respectively). f Representative FACS plots showing conversion of eGFP– B cells from NOD.Il10-eGFP donors into eGFP+/CD5+/CD1dhi Bregs in PDC-E2166–181/IAg7-NP-treated hosts. g Percentages of B cell-to-Breg cell conversion in PDC-E2166–181/IAg7-NP vs. Cys-NP-treated NOD.c3c4 hosts, in liver, spleen, PCLNs, and MLNs (n = 3 and 4 mice/treatment type, respectively). h Percentages of B cell-to-Breg cell conversion in PDC-E2166–181/IAg7-NP-treated NOD.c3c4 hosts, in spleen, PCLNs, and MLNs, as a function of the peptide displayed on the B-cell surface (non-cognate: BDC2.5mi, n = 4/organ; or cognate: PDC166–181, n = 3/organ). Data correspond to mean ± SEM. P values were calculated via multiple t-test analysis (a and b), Mann–Whitney U (c, e, g and h) or Pearson’s correlation test (d)
