Fig. 8

H2A.Z is an effector of the Wnt pathway on differentiation markers. a Chromatin from sub-confluent CRISPR-Cas9-modified HCT116 cells to homozygously delete the major TCF7L2 binding region of the H2AFZ gene promoter, using indicated H2AFZ promoter—specific or intergenic sequence—targeting primers. Results and standard errors (n = 3 technical replicates) are shown for two independent experiments. b Control or CRISPR-modified HCT116 cells were transfected using β-catenin—targeting or control siRNA. 72 h later, total RNA was extracted and subjected to RT-qPCR analysis for expression of the indicated genes (calculated relative to GAPDH and β-actin mRNA levels). The mean and standard errors are shown (n = 4 independent experiments). The Student’s t-Test p-value was calculated (for β-catenin -depleted samples from control vs CRISPR-modified cells) and is indicated for each gene