Fig. 2
From: Highly sensitive CE-ESI-MS analysis of N-glycans from complex biological samples
Extracted ion electropherograms of derivatized and labelled N-glycans. a Extracted ion electropherograms of two N-glycan standards (H3N4 and H5N4) after injection of a final amount of 20 amol. b, c Illustration of the obtained isotopic pattern and mass accuracy (blue trace) for the doubly charged species H3N4 and H5N4 compared with the theoretical isotopic pattern (orange trace). d Extracted ion electropherograms of the 10 most abundant N-glycans (>2% relative abundance) present in TPNG, e 10 medium abundant N-glycans (between 0.5–1.0% relative abundance) and f 10 low abundant N-glycans (<0.25% relative abundance). Separation was achieved using a bare-fused silica capillary after a dynamic neutral coating. *Indicates in-source decay of the H6N2. # Shows the presence of doubly charged species of H5N5F1 of which the m/z values overlap with the ones of the triply charged species of H6N5F1S2,32S2,61. Blue square: N-acetylglucosamine, green circle: mannose, yellow circle: galactose, red triangle: fucose, right pointing pink diamond: α2,6-linked N-acetylneuraminic acid, left pointing pink diamond: α2,3-linked N-acetylneuraminic acid, GirP: Girard’s reagent P label
