Fig. 3
From: Highly sensitive CE-ESI-MS analysis of N-glycans from complex biological samples
CE-ESI-MS/MS fragmentation spectra of derivatized and labelled N-glycans from TPNG. The following precursor ions were fragmented: a 760.9603+ (H4N5F1S2,61), b 1148.9392+ (H5N5S2,61) and c 1072.0763+ (H6N5F1S2,31S2,62). The Y-ion fragmentation pattern is annotated in blue and the B-ion fragmentation pattern is annotated in orange. Blue diamond: precursor ion, blue square: N-acetylglucosamine, green circle: mannose, yellow circle: galactose, red triangle: fucose, right pointing pink diamond: α2,6-linked N-acetylneuraminic acid, left pointing pink diamond: α2,3-linked N-acetylneuraminic acid, GirP: Girard’s reagent P label. *The presence of an antennary fucose in combination with an α2,6-linked sialic acid is likely, for a large part, due to fucose migration during MS analysis36
