Fig. 4
From: Development and application of a high-content virion display human GPCR array
Binding kinetics of SRIF-14 to SSTR2, K082, and other three virions. a Principle of binding kinetics measurement using virion-oscillator device. b–f Binding kinetics of SRIF-14 to its canonical receptor, SSTR2, and three newly discovered off-target GPCRs. The red and purple arrows mark the starting time points of the association and the dissociation phases, respectively. Binding curves were fit using the first order kinetics model (solid lines). The calculated affinity values range from 11.2 nM (SSTR2) to 25.0 µM (KISS1R), while K082 virion showed no detectable binding activity to the SRIF-14. Detailed ka, kd, and KD values are listed in Supplementary Table 1
