Fig. 2

Phosphorylation hotspots overlapping with human phosphosites of known function. For 4 protein domain families we show the enrichment over random of protein phosphorylation along the domain sequence. The average number of phosphosites observed per rolling window is plotted in a solid black line (observed). The background level of expected phosphorylation calculated from random sampling is shown in gray line, with standard deviations as gray band. The blue line represents the negative logarithm of p-value at each position (right y axis). A horizontal red line indicates a cut-off of the Bonferroni corrected p-value of 0.01. Positions with a −log(p-value) above this cut-off and average phosphosites per window higher than 2 are considered putative regulatory regions and highlighted under a vertical yellow bar. Red circles indicate human phosphosite positions with known regulatory function. In the structural representations the predicted hotspot regions are highlighted in yellow