Fig. 3

Structural features of phosphorylation hotspots. a Enrichment over random of structural features comparing hotspots with other residues within the same domains. The tested features include water accessibility—residues with >20% RSA; protein disorder as predicted by DISOPRED; catalytic residues; residues within 5 amino-acid distance to a catalytic residue; residues within 5 Å of a catalytic residue; residues at an interface based on 3DID. For each feature we report the −log(p-value), p-value calculated using Fisher’s exact test. b Examples of hotspot regions at interfaces where the hotspot region (red) from a domain (gray) has been observed contacting many other types of domains (other colors) in empirical structures