Fig. 4
From: Conserved phosphorylation hotspots in eukaryotic protein domain families
Examples of putative regulatory hotspots at or near catalytic residues. The average number of phosphosites observed per rolling window is plotted in a solid black line (observed). The background level of randomly expected phosphorylation is shown in gray line, with standard deviations as gray band. The blue line represents the negative logarithm of p-value at each position (right y axis). A horizontal red line indicates a cut-off of the Bonferroni corrected p-value of 0.05. Positions with a −log(p-value) above this cut-off and average phosphosites per window higher than 2 are considered putative regulatory regions and highlighted under the yellow bar. Red circles indicate human phosphosite positions with known regulatory function and yellow circles represent catalytic residue positions. In the structural representations the predicted hotspot regions are highlighted in yellow. The catalytic residues have been represented in as orange sticks and in red stick representations are substrates or products
