Fig. 5

Hotspot regions near catalytic residues that are distal in protein sequence. a The IMPDH hotspot region is represented in yellow segment. In the insets, the loop near the hotspot region is shown changing from an open conformation (blue volumes) to closed conformation (magenta volumes). A serine residue within the hotspot region (yellow sticks) points to substrate binding pocket and is often found phosphorylated across species (see alignment). b The transaldolase hotspot region is shown in yellow. In the structural inset a serine within the hotspot region (yellow sticks) is found just at the entrance of the substrate cavity. The identified phosphorylation sites contributing to the identification of the hotspot region are shown in the alignments in red