Fig. 6
From: Conserved phosphorylation hotspots in eukaryotic protein domain families
Rps14a T119A mutant shows growth and 20S processing defects in cold shock. a The phosphorylation enrichment over random for the ribosomal S11 domain (PFAM:PF00411) is plotted in a solid black line. The background expectation is shown in gray line, with standard deviations as gray band. The blue line represents the negative logarithm of p-value (Y axis on the right side). A horizontal red line indicates a cut-off equivalent to a Bonferroni corrected p-value of 0.05. Mutated residues in Rps14a (T119 and S123) are indicated by orange stars in the plot and shown in b as orange stick representations (PDB:5wnt_K). c Conservation of phosphorylation sites in this region across species. d Growth curve for Rps14a T119A and S123A mutants in 25 °C in SC media. Source data are provided as a Source Data file. e In situ hybridization with a Cy3-labeled oligonucleotide complementary to the 5′ sequence portion of ITS1 was assayed in 30 and 20 °C. f Structural representation of contacts between the hotspot region of Rps14a (represented in gray) and the ATPase domain of Fap7 (represented in orange)
