Fig. 4
From: High-resolution specificity profiling and off-target prediction for site-specific DNA recombinases
Rec-seq profiles of Dre, VCre, and Bxb1 site-specific recombinases. a Cre, Dre, and VCre differ at the protein sequence level, and bind different recognition targets. b Rec-seq of tyrosine recombinases Dre and VCre. Values represent the geometric mean of n = 3 independent replicates (dots) conducted at 37 °C for 30 min at a 3:1 protein:DNA ratio. c Heat map of Rec-seq enrichment values for VCre showing the log2 of the enrichment value for each nucleotide at each position in loxV relative to the canonical base (black outline). d Rec-seq of serine integrase Bxb1 on its substrates attP and attB. Both substrates contain a conserved ACNAC motif (gray box). Values represent the geometric mean of n = 3 independent replicates (dots) conducted at 37 °C for 30 min at a 3:1 protein:DNA ratio. Source data are provided as a Source Data file
